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real time pcr system  (Roche)


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    Structured Review

    Roche real time pcr system
    Real Time Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lightcycler+real+time+pcr+system/LightCycler+480+System/pmc12996981-51-18-21
    Average 99 stars, based on 6 article reviews
    real time pcr system - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Genome-wide discovery and phenotyping of non-coding transcripts in A. fumigatus reveals lncRNAs with a role in antifungal drug sensitivity
    Article Snippet: Concentration and purity of total RNA and cDNAs were assessed using a NanoDrop Lite spectrophotometer (Thermo Scientific, USA). qPCRs were performed in a 10 μL final volume containing 10 ng of cDNA, 3 pmol of each primer, and 5 μL of iTaqTM Universal SYBR® Green Supermix (2X). .. Reactions were run on a Roche LightCycler® real-time PCR system with the following cycling conditions: 40 cycles of 15 seconds at 95 °C, 30 seconds at 60 °C, and 30 seconds at 72 °C. ..

    Article Title: Herbal Melanin Inhibits Colorectal Cancer Cell Motility, Invasiveness, and Epithelial–Mesenchymal Transition, Associated with u-PAR Downregulation Through JNK and ERK Pathways
    Article Snippet: .. PCR samples were generated using an iScript One-step RT-PCR kit with SYBR ® Green (#1725120, Bio-Rad Laboratories) and visualized on a LightCycler ® real-time PCR system (Roche Diagnostics, Basel, Switzerland) [ ]. ..

    Article Title: Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers.
    Article Snippet: RT-PCR was conducted using Go-Taq (Promega, USA) and the GeneAmp RNA PCR system (Applied Biosystems, USA), with GAPDH serving as the internal reference. .. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR Green (Thermo Fisher, USA) on the LightCycler real-time PCR system (Roche, CH), also referencing GAPDH. ..

    Article Title: The overall and sequence-specific degradation of soil extracellular 16S rRNA genes across China: rates and influential factors
    Article Snippet: Finally, DNA was extracted from these precipitates using the DNeasy PowerSoil kit (Qiagen, Hilden, Germany) following manufacturer’s protocols. .. The copy numbers of 16S rRNA genes were determined using a LightCycler real-time PCR System (Roche, Germany). ..

    Article Title: Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers
    Article Snippet: RT-PCR was conducted using Go-Taq (Promega, USA) and the GeneAmp RNA PCR system (Applied Biosystems, USA), with GAPDH serving as the internal reference. .. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR Green (Thermo Fisher, USA) on the LightCycler real-time PCR system (Roche, CH), also referencing GAPDH. ..

    Article Title: BMAL1 attenuates myocardial infarction-induced fibrosis via suppressing p-SMAD3/SMAD3 in TGF-β1 pathway
    Article Snippet: Complementary DNA (cDNA) was synthesized using the PrimeScript RT Master Mix (RR036A, Takara, Japan). .. RT-qPCR was carried out in the LightCycler® Real-Time PCR System (Roche, USA) using SYBR Master Mix (Vazyme, China). ..

    Article Title: 5-hydroxymethylfurfural as an important component of cigarette smoke impairs the blood-testis barrier via mTOR/Atg12-mediated autophagy in Sertoli cells in mice.
    Article Snippet: RNA from testicular tissues was extracted with TRIzol reagent and reverse-transcribed into complementary DNA (cDNA) using HiScript II qRT SuperMix (Vazyme, China). .. The cDNA products were subsequently amplified with ChamQ SYBR qPCR Master Mix (Vazyme, China) on a LightCycler real-time PCR system (Roche, Switzerland) under the following conditions: initial activation at 95◦C for 30 s, followed by 40 cycles of denaturation at 95◦C for 10 s and annealing at 60◦C for 30 s. Gene-specific primer sequences are listed in Table S2. ..

    Article Title: Genome-wide discovery and phenotyping of non-coding transcripts in A. fumigatus reveals lncRNAs with a role in antifungal drug sensitivity.
    Article Snippet: Concentration and purity of total RNA and cDNAs were assessed using a NanoDrop Lite spectrophotometer (Thermo Scientific, USA). qPCRs were performed in a 10μL final volume containing 10 ng of cDNA, 3 pmol of each primer, and 5μL of iTaqTM Universal SYBR® Green Supermix (2X). .. Reactions were run on a Roche LightCycler® real-time PCR system with the following cycling conditions: 40 cycles of 15 seconds at 95 °C, 30 seconds at 60 °C, and 30 seconds at 72 °C. ..

    Polymerase Chain Reaction:

    Article Title: Herbal Melanin Inhibits Colorectal Cancer Cell Motility, Invasiveness, and Epithelial–Mesenchymal Transition, Associated with u-PAR Downregulation Through JNK and ERK Pathways
    Article Snippet: .. PCR samples were generated using an iScript One-step RT-PCR kit with SYBR ® Green (#1725120, Bio-Rad Laboratories) and visualized on a LightCycler ® real-time PCR system (Roche Diagnostics, Basel, Switzerland) [ ]. ..

    Article Title: Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers.
    Article Snippet: RT-PCR was conducted using Go-Taq (Promega, USA) and the GeneAmp RNA PCR system (Applied Biosystems, USA), with GAPDH serving as the internal reference. .. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR Green (Thermo Fisher, USA) on the LightCycler real-time PCR system (Roche, CH), also referencing GAPDH. ..

    Article Title: Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers
    Article Snippet: RT-PCR was conducted using Go-Taq (Promega, USA) and the GeneAmp RNA PCR system (Applied Biosystems, USA), with GAPDH serving as the internal reference. .. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR Green (Thermo Fisher, USA) on the LightCycler real-time PCR system (Roche, CH), also referencing GAPDH. ..

    Generated:

    Article Title: Herbal Melanin Inhibits Colorectal Cancer Cell Motility, Invasiveness, and Epithelial–Mesenchymal Transition, Associated with u-PAR Downregulation Through JNK and ERK Pathways
    Article Snippet: .. PCR samples were generated using an iScript One-step RT-PCR kit with SYBR ® Green (#1725120, Bio-Rad Laboratories) and visualized on a LightCycler ® real-time PCR system (Roche Diagnostics, Basel, Switzerland) [ ]. ..

    One Step RT-PCR:

    Article Title: Herbal Melanin Inhibits Colorectal Cancer Cell Motility, Invasiveness, and Epithelial–Mesenchymal Transition, Associated with u-PAR Downregulation Through JNK and ERK Pathways
    Article Snippet: .. PCR samples were generated using an iScript One-step RT-PCR kit with SYBR ® Green (#1725120, Bio-Rad Laboratories) and visualized on a LightCycler ® real-time PCR system (Roche Diagnostics, Basel, Switzerland) [ ]. ..

    SYBR Green Assay:

    Article Title: Herbal Melanin Inhibits Colorectal Cancer Cell Motility, Invasiveness, and Epithelial–Mesenchymal Transition, Associated with u-PAR Downregulation Through JNK and ERK Pathways
    Article Snippet: .. PCR samples were generated using an iScript One-step RT-PCR kit with SYBR ® Green (#1725120, Bio-Rad Laboratories) and visualized on a LightCycler ® real-time PCR system (Roche Diagnostics, Basel, Switzerland) [ ]. ..

    Article Title: Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers.
    Article Snippet: RT-PCR was conducted using Go-Taq (Promega, USA) and the GeneAmp RNA PCR system (Applied Biosystems, USA), with GAPDH serving as the internal reference. .. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR Green (Thermo Fisher, USA) on the LightCycler real-time PCR system (Roche, CH), also referencing GAPDH. ..

    Article Title: Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers
    Article Snippet: RT-PCR was conducted using Go-Taq (Promega, USA) and the GeneAmp RNA PCR system (Applied Biosystems, USA), with GAPDH serving as the internal reference. .. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR Green (Thermo Fisher, USA) on the LightCycler real-time PCR system (Roche, CH), also referencing GAPDH. ..

    Reverse Transcription:

    Article Title: Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers.
    Article Snippet: RT-PCR was conducted using Go-Taq (Promega, USA) and the GeneAmp RNA PCR system (Applied Biosystems, USA), with GAPDH serving as the internal reference. .. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR Green (Thermo Fisher, USA) on the LightCycler real-time PCR system (Roche, CH), also referencing GAPDH. ..

    Article Title: Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers
    Article Snippet: RT-PCR was conducted using Go-Taq (Promega, USA) and the GeneAmp RNA PCR system (Applied Biosystems, USA), with GAPDH serving as the internal reference. .. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR Green (Thermo Fisher, USA) on the LightCycler real-time PCR system (Roche, CH), also referencing GAPDH. ..

    Quantitative RT-PCR:

    Article Title: BMAL1 attenuates myocardial infarction-induced fibrosis via suppressing p-SMAD3/SMAD3 in TGF-β1 pathway
    Article Snippet: Complementary DNA (cDNA) was synthesized using the PrimeScript RT Master Mix (RR036A, Takara, Japan). .. RT-qPCR was carried out in the LightCycler® Real-Time PCR System (Roche, USA) using SYBR Master Mix (Vazyme, China). ..

    Amplification:

    Article Title: 5-hydroxymethylfurfural as an important component of cigarette smoke impairs the blood-testis barrier via mTOR/Atg12-mediated autophagy in Sertoli cells in mice.
    Article Snippet: RNA from testicular tissues was extracted with TRIzol reagent and reverse-transcribed into complementary DNA (cDNA) using HiScript II qRT SuperMix (Vazyme, China). .. The cDNA products were subsequently amplified with ChamQ SYBR qPCR Master Mix (Vazyme, China) on a LightCycler real-time PCR system (Roche, Switzerland) under the following conditions: initial activation at 95◦C for 30 s, followed by 40 cycles of denaturation at 95◦C for 10 s and annealing at 60◦C for 30 s. Gene-specific primer sequences are listed in Table S2. ..

    Activation Assay:

    Article Title: 5-hydroxymethylfurfural as an important component of cigarette smoke impairs the blood-testis barrier via mTOR/Atg12-mediated autophagy in Sertoli cells in mice.
    Article Snippet: RNA from testicular tissues was extracted with TRIzol reagent and reverse-transcribed into complementary DNA (cDNA) using HiScript II qRT SuperMix (Vazyme, China). .. The cDNA products were subsequently amplified with ChamQ SYBR qPCR Master Mix (Vazyme, China) on a LightCycler real-time PCR system (Roche, Switzerland) under the following conditions: initial activation at 95◦C for 30 s, followed by 40 cycles of denaturation at 95◦C for 10 s and annealing at 60◦C for 30 s. Gene-specific primer sequences are listed in Table S2. ..



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